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Extraction
Summary
- Animal sample (Should be less than 10 mg.)
- GenCheck 40 ul
- High speed Centrifuge at 10,000 rpm for 30 seconds.
- Thermo-shaker at 98-100 ° C for 10 minutes.
- Stay on ice for 1 minute.
- Centrifuge at 10,000 rpm for 5 minutes.
- Transfer 5μl of the supernatant and add 95μl of RNase-free water.
Amplification
For Sample
- PCR enzyme Primer 5μl per sample
- RNase-free water 2μl per sample
- Primer Solution 2μl per sample
- Extracted DNA 1μl
- Micropipette
- Centrifuge
- Thermocycler
For Positive Controls and Negative Controls:
- PCR enzyme Primer 5 ul x (Number of Positive Controls available in the Test Kit)
- RNase-free Water 2μl x (Number of Positive Controls available in the Test Kit)
- Primer Solution 2μl x (Number of Positive Controls available in the Test Kit)
- For Positive Control(s): Put 1μl of Positive Control for each disease (EHP, WSSV, IHHNV, AHPND/EMS).
- For Negative Control: Put 1μl of RNAse-Free Water.
- Micropipette
- Microcentrifuge (using Multi Spin low speed)
- Thermocycler (ASTEC 325 GeneAtlas PCR machine or other equipment recommended).
Identification
For Sample
- Latex Solution 1.5μl
- Dilution Buffer 20μl
- Positive Controls 1μl
- Amplified DNA 1μl
- Micropipette
- C-PAS Strip (1 Strip per sample)