{"id":2029,"date":"2022-02-04T11:39:37","date_gmt":"2022-02-04T11:39:37","guid":{"rendered":"http:\/\/www.sure-bio.com\/process-flow-of-pcr-analysis-with-dna-test-kits-copy\/"},"modified":"2022-02-05T11:22:20","modified_gmt":"2022-02-05T11:22:20","slug":"process-flow-of-pcr-analysis-with-dna-test-kits-copy","status":"publish","type":"page","link":"http:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits-copy\/","title":{"rendered":"FLUJO DE PROCESO DE ANALISIS DE PCR CON KITS DE PRUEBA DE ADN - Procedimiento Paso a Paso"},"content":{"rendered":"<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 bg-primary d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;\"><strong>Extracci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<ul><li class=\" translation-block\">Muestra de animal (menos de 10mg) \u2013 Homogeneizar.<\/li><li class=\" translation-block\">Agregue 40\u03bcl de GenCheck.<\/li><li><strong>V\u00f3rtice.<\/strong><\/li><li><strong>Centrifugar a 10000rpm durante 30 segundos todas las muestras.<\/strong><\/li><li class=\" translation-block\">Colocar las muestras en el Bloque t\u00e9rmico (agitador t\u00e9rmico) a 98-100\u00b0C durante 10 minutos.<\/li><li class=\" translation-block\">Permanecer en hielo durante 1 minuto.<\/li><li><strong>Centrifugar a 10000rpm durante 5 minutos.<\/strong><\/li><li class=\" translation-block\">Transfiera 5\u03bcl de sobrenadante de las muestras a un nuevo tubo de 1.5 ml.<\/li><li class=\" translation-block\">Agregue 95\u03bcl de agua libre de RNasa a cada muestra.<\/li><\/ul>\n\n\n\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;background-color:#28a745\"><strong>Amplificaci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<h3>Para Muestra<\/h3>\n\n\n\n<ul><li class=\" translation-block\">Prepare la mezcla de PCR en un tubo de 1.5 ml de la siguiente manera:<\/li><\/ul>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container\">\n<p class=\" translation-block\">PCR enzima 5\u03bcl X<br \/>RNase free water 2\u03bcl X<br \/>Primer Solution 2\u03bcl X.<\/p>\n\n\n\n<ul><li class=\" translation-block\">Lleve la mezcla de PCR al vortex.<\/li><li class=\" translation-block\">Coloque 9\u03bcl (mezcla de PCR) en cada tubo de PCR.<\/li><li class=\" translation-block\">Coloque 1\u03bcl de muestra de ADN o control positivo en cada tubo de PCR.<\/li><li><strong>Lleve de Nuevo la mezcla al Multispin.<\/strong>.<\/li><li class=\" translation-block\">Colocar las muestras en el Termociclador de PCR.<\/li><\/ul>\n\n\n\n<p><strong><span style=\"text-decoration: underline;\"><em><span class=\"has-inline-color has-vivid-red-color\">MUY IMPORTANTE Notas:<\/span><\/em><\/span><\/strong><\/p>\n\n\n\n<ul><li class=\" translation-block\">El conjunto enfriador (PCR Cooler set), la enzima de PCR y el control positive deben congelarse (almacenar a - 20\u00b0C )<\/li><li class=\" translation-block\">Todos los reactivos, por ejemplo, el agua sin ARNasa y las soluciones de cebador, DEBEN SER REFRIGERADOS (almacenados entre +2 \u00b0C y +8 \u00b0C) o en temperatura FR\u00cdA<\/li><li class=\" translation-block\">Coloque las rejillas para tubos de 1.5 o 2.0 ml encima de una bolsa de hielo durante el uso.<\/li><\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;background-color:#ff7907\"><strong>Identificaci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<h3>IDENTIFICACI\u00d3N de los pat\u00f3genos del camar\u00f3n:<\/h3>\n\n\n\n<ul><li class=\" translation-block\">Prepare la mezcla de latex (en un tubo de 1.5ml):<\/li><\/ul>\n\n\n\n<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  <strong>Soluci\u00f3n de latex&nbsp;&nbsp;&nbsp;&nbsp;        <span class=\"has-inline-color has-vivid-red-color\">1.5ul<\/span><\/strong> X<br>    &nbsp;&nbsp;&nbsp;&nbsp;  <strong>Dilution Buffer&nbsp;&nbsp;&nbsp;&nbsp;<span class=\"has-inline-color has-vivid-red-color\">   20ul<\/span><\/strong> X<\/p>\n\n\n\n<ul><li class=\" translation-block\">Lleve la mezcla de latex al Multispin<\/li><li class=\" translation-block\">Ponga 21.5\u03bcl de la mezcla de latex en un tubo despues de la PCR.<\/li><li class=\" translation-block\">Despues de esto, coloque una tira C-PAS en cada tubo de PCR.<\/li><li class=\" translation-block\">Espere 10 minutos.<\/li><li class=\" translation-block\">Y finalmente, IDENTIFIQUE visualmente los PAT\u00d3GENOS DEL CAMAR\u00d3N que aparecen como l\u00edneas AZULES en la tira C-PAS o colocando la(s) tira(s) C-PAS en la tarjeta C-PAS o el LECTOR de im\u00e1genes.<\/li><\/ul>\n\n\n\n<p><\/p>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Extraction Obtain SAMPLE [For Animal (Shrimp) sample, use less than 10mg.] &#8212;&gt; HOMOGENIZE manually. Add 40ul of the GenCheck extraction Reagent. Vortex. Centrifuge at 10000 rpm for 30 seconds. Heat block (Thermo-Shaker) at 98-100 \u00b0C for 10 minutes. Stay on ice for 1 minute. Centrifuge at 10000 rpm for 5 minutes. Transfer 5 ul of<a class=\"moretag\" href=\"http:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits-copy\/\"><span class=\"screen-reader-text\">Read more about Process Flow of PCR Analysis with DNA Test Kits &#8211; Step by Step Procedure<\/span>[&#8230;]<\/a><\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Process Flow of PCR Analysis with DNA Test Kits - Step by Step Procedure &ndash; SureBio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits-copy\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Process Flow of PCR Analysis with DNA Test Kits - Step by Step Procedure &ndash; SureBio\" \/>\n<meta property=\"og:description\" content=\"Extraction Obtain SAMPLE [For Animal (Shrimp) sample, use less than 10mg.] &#8212;&gt; HOMOGENIZE manually. 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