{"id":1994,"date":"2022-02-04T10:47:42","date_gmt":"2022-02-04T10:47:42","guid":{"rendered":"http:\/\/www.sure-bio.com\/?page_id=1994"},"modified":"2022-02-04T11:39:07","modified_gmt":"2022-02-04T11:39:07","slug":"process-flow-of-pcr-analysis-with-dna-test-kits","status":"publish","type":"page","link":"http:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits\/","title":{"rendered":"FLUJO DE PROCESO DE ANALISIS DE PCR CON KITS DE PRUEBA DE\nADN - Sumario"},"content":{"rendered":"<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 bg-primary d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;\"><strong>Extracci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<h3>Sumario<\/h3>\n\n\n\n<ul><li class=\" translation-block\">Muestra de animal (menos de 10mg)<\/li><li class=\" translation-block\">GenCheck 40 \u03bcl<\/li><li><strong>High speed<\/strong> Centrifuge at <strong>10,000 rpm<\/strong> <font color=\"#000000\"><u><strong>CONFIRMATORIO <\/strong><\/u><\/font> <strong>30 seconds<\/strong>.<\/li><li>Thermo-shaker at <strong>98-100 \u00b0 C<\/strong> <font color=\"#000000\"><u><strong>CONFIRMATORIO <\/strong><\/u><\/font> <strong>10 minutes<\/strong>.<\/li><li class=\" translation-block\">Permanecer en hielo durante 1 minuto.<\/li><li>Centrifuge at <strong>10,000 rpm<\/strong> <font color=\"#000000\"><u><strong>CONFIRMATORIO <\/strong><\/u><\/font> <strong>5 minutes<\/strong>.<\/li><li class=\" translation-block\">Transfiera 5\u03bcl del sobrenadante y agregue 95\u03bcl de agua libre de RNasa.<\/li><\/ul>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;background-color:#28a745\"><strong>Amplificaci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<h3>Para Muestra<\/h3>\n\n\n\n<ul><li class=\" translation-block\">Primer PCR enzyme 5\u03bcl x muestra<\/li><li class=\" translation-block\">RNase-free water 2\u03bcl x muestra<\/li><li class=\" translation-block\">Primer Solution 2\u03bcl x muestra<\/li><li><strong>Extracted<\/strong> <strong>virus de ADN <\/strong> <strong>1\u03bcl<\/strong><\/li><li>Micropipetas<\/li><li>Centrifuga<\/li><li>Termociclador<\/li><\/ul>\n\n\n\n<h3>Para Controle s Positivos y Controles Negativos:<\/h3>\n\n\n\n<ul><li class=\" translation-block\"><strong>Primer PCR enzyme<\/strong> 5\u03bcl x (N\u00famero de Controles Positivos disponibles en el kit de prueba)<\/li><li class=\" translation-block\"><strong>RNAse-Free Water<\/strong> 2\u03bcl x (N\u00famero de Controles Positivos disponibles en el kit de prueba)<\/li><li class=\" translation-block\"><strong>Primer Solution<\/strong> 2\u03bcl x (N\u00famero de Controles Positivos disponibles en el kit de prueba)<\/li><li><strong>For Positive Control(s):<\/strong> Put <strong>1\u03bcl<\/strong> of <strong>Positive Control<\/strong> <font color=\"#000000\"><u><strong>CONFIRMATORIO <\/strong><\/u><\/font> <strong>each disease<\/strong> (EHP, WSSV, IHHNV, AHPND<strong>\/<\/strong>EMS).<\/li><li class=\" translation-block\"><strong>Para Control Negativo:<\/strong> Ponga 1\u03bcl de RNAse-Free Water.<\/li><li>Micropipetas<\/li><li class=\" translation-block\">Minicentrifuga (usando el Multi Spin de baja velocidad)<\/li><li class=\" translation-block\">Termociclador (Quick Bath QB-0224B o otro equipo de PCR recomendado).<\/li><\/ul>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\" style=\"flex-basis:33.33%\">\n<h4 class=\"has-text-align-center d-flex w-100 h-100 d-flex align-items-center\" style=\"height:100%;width:100%;vertical-align:center;align-items:center;justify-content:center;display:flex; color:#fff;letter-spacing:1px;margin:0;padding:0;background-color:#ff7907\"><strong>Identificaci\u00f3n<\/strong><\/h4>\n<\/div>\n\n\n\n<div class=\"wp-block-column\" style=\"flex-basis:66.66%\">\n<h3>Para Muestra<\/h3>\n\n\n\n<ul><li>Latex Solution <strong>1.5\u03bcl<\/strong><\/li><li>Dilution Buffer <strong>20\u03bcl<\/strong><\/li><li class=\" translation-block\">Controles positivos <strong>1\u03bcl<\/strong><\/li><li><strong><strong>Amplified<\/strong> <strong>virus de ADN <\/strong> <strong>1\u03bcl<\/strong><\/strong><\/li><li>Micropipetas<\/li><li class=\" translation-block\"><strong>C-PAS<\/strong> (<strong>1Tirilla x muestra<\/strong>)<\/li><\/ul>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Extraction Summary Animal sample (Should be less than 10 mg.) GenCheck 40 ul High speed Centrifuge at 10,000 rpm for 30 seconds. Thermo-shaker at 98-100 \u00b0 C for 10 minutes. Stay on ice for 1 minute. Centrifuge at 10,000 rpm for 5 minutes. Transfer 5\u03bcl of the supernatant and add 95\u03bcl of RNase-free water. Amplification<a class=\"moretag\" href=\"http:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits\/\"><span class=\"screen-reader-text\">Read more about Process Flow of PCR Analysis with DNA Test Kits &#8211; Summary<\/span>[&#8230;]<\/a><\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Process Flow of PCR Analysis with DNA Test Kits - Summary &ndash; SureBio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sure-bio.com\/es\/process-flow-of-pcr-analysis-with-dna-test-kits\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Process Flow of PCR Analysis with DNA Test Kits - Summary &ndash; SureBio\" \/>\n<meta property=\"og:description\" content=\"Extraction Summary Animal sample (Should be less than 10 mg.) GenCheck 40 ul High speed Centrifuge at 10,000 rpm for 30 seconds. Thermo-shaker at 98-100 \u00b0 C for 10 minutes. Stay on ice for 1 minute. Centrifuge at 10,000 rpm for 5 minutes. Transfer 5\u03bcl of the supernatant and add 95\u03bcl of RNase-free water. 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GenCheck 40 ul High speed Centrifuge at 10,000 rpm for 30 seconds. Thermo-shaker at 98-100 \u00b0 C for 10 minutes. Stay on ice for 1 minute. Centrifuge at 10,000 rpm for 5 minutes. Transfer 5\u03bcl of the supernatant and add 95\u03bcl of RNase-free water. 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